The nuptials come just five months after the glittering wedding of Prince Harry, younger son of heir-to-the-throne Prince Charles, and USA actress wife Meghan, now the Duchess of Sussex, at the same location.
And while everyone is comparing Eugenie's wedding to Prince Harry and Meghan's lavish affair, some royal fanatics have noticed the similarity between Eugenie's and Catherine's engagement rings. Princess Eugenie, the younger daughter of Prince Andrew and Sarah Ferguson, is set to Wednesday Jack Brooksbank in a two-day extravaganza, marking another highly-anticipated union to take place at St George's Chapel at Windsor Castle.
Following the ceremony, the newlyweds will take part in a royal carriage procession through Windsor.
Her cousin Prince Harry and wife Meghan had their glittering wedding in May at the same location.
Other bridesmaids include Maud Windsor, 5, the daughter of Eugenie's goddaughter and the daughter of Lady Frederick Windsor and Lord Frederick Windsor, and Miss Theodora Williams, the daughter Ayda Field and "X Factor" host Robbie Williams.
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The Duchess of Cornwall Camila is another family member who will not be able to attend Eugenie's actual wedding on Friday due to a preexisting roster of official duties.
It is not yet known whether Prince Philip will attend the ceremony. He and Eugenie have dated for many years.
The Queen and the Duke of Edinburgh are in residence at Windsor Castle ahead of their granddaughter's wedding tomorrow.
The presence of so many royals - and a number of celebrity guests - has prompted extra security measures to be put in place.
If you don't have time to watch the whole programme, don't worry.
The pair are set to walk down the aisle at St George's Chapel in Windsor Castle, just like Prince Harry and Meghan Markle did on May 19.