All in all, including the urban cases, 14 cases of Ebola have been confirmed according to the reports from the Congo Health Ministry.
The World Health Organisation (WHO) is convening an emergency meeting on Friday over the latest outbreak of the deadly Ebola disease in the Democratic Republic of the Congo (DRC).
Until now, the outbreak had been confined to remote rural areas, where Ebola, which is spread via contact with bodily fluids of those infected, travels more slowly.
One sample proved positive for Ebola, he said.
Mbandaka, a city of nearly 1.2 million, is in a busy travel corridor in Congo's northwest Equateur province and is upstream from the capital, Kinshasa, a city of about 10 million. "There is a fridge that will be prepared (on Thursday) ... in Mbandaka and that will be at -80°".
"The arrival of Ebola in an urban area is very concerning and WHO and partners are working together to rapidly scale up the search for all contacts of the confirmed case in the Mbandaka area", WHO Regional Director for Africa, Matshidiso Moeti, said in a statement.
Healthcare teams fear that the spread of the infection would be faster in the city compared to the rural areas that they were dealing with up until now.
The aid group Medecins Sans Frontieres said 514 people who are thought to have been in contact with infected people were now being monitored.
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Congo's health minister Oly Ilunga signalled on Thursday that his government was fully behind the shot's use.
Congolese Health Ministry officials carry the first batch of experimental Ebola vaccines in Kinshasa, Democratic Republic of Congo May 16, 2018.
"This is a concerning development, but we now have better tools than ever before to combat Ebola", said Tedros Adhanom Ghebreyesus, the Director-General of WHO.
Ebola survivors often suffer from long-term consequences of the disease and may carry dormant viruses.
Ebola has no known cure and no fully effective vaccine. The rituals around traditional burial practices are an issue that "always draws a lot of attention and reticence and complication", he said.
The Department of Health first reported the outbreak on May 8, the ninth the country has faced in the past four decades.
Naija News reports that earlier in the week, World Health Organization delivered 5,000 Ebola vaccines in Congo as part of the UN's efforts to control the spread of the disease. Whilst the strategy is being put in place, the "pillars" of an Ebola intervention are taking effect in order to stem the spread of the disease: early treatment and isolation of people who are sick; tracing and following up contacts; informing people about the disease, how to prevent it and where to seek care; supporting existing healthcare; and temporarily changing cultural behaviour around funerals.
First symptoms generally include the sudden onset of fever, fatigue, muscle pain, headache and sore throat.